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Home > Products > Click chemistry> DBCO Reagents> DBCO-PEG4-Desthiobiotin
DBCO-PEG4-Desthiobiotin
Catalog # Pkg Size Price Quantity Shopping Cart
R-CD-030 10mg ¥ 2384
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R-CD-030 25mg ¥ 4464
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結構式
外觀 Colorless to slightly yellow oil
分子量 719.87
溶解度 DMSO, DMF, DCM, THF, Chloroform
CAS號 N/A
質量控制 95%
儲存條件 -20℃
保存時間 1 year
其他信息 N/A
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
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